Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflow

Presently, the attention given to natural substances counteract damage produced by oxidative stress (OS) has risen sharply. In this scenario, hydroxytyrosol (HT) derivatives, formed as a result of HT conjugation with fatty acids (FAs) (lipophenols), have been recently described in foodstuffs such extra virgin olive oil, being powerful bioactive compounds higher activity than unesterified phenolic compound. The present work describes capacity lipophenols act on course OS and secondary inflammatory processes, based their modulate isoprostanoid profile induced H2O2 THP-1 monocytic cells. A UHPLC-QqQ-ESI-MS/MS-based lipidomics workflow was applied over range 37 human oxylipins. main outcomes retrieved suggest both HT-lipophenols regulators cellular redox balance, acting pro-oxidants vitro, which is highly dependent experimental conditions. Our anti-inflammatory potential HT-lipophenols, where type FAs core appears be critical for defining bioactivity lipophenols, highlighting that lipidomic approach, simultaneous analysis multiple oxylipins, understanding generation hence, pathophysiological processes.